Shihui Dong

Ph.D., Professor


Prof. Shihui Dong received his B.S. degree from College of Pharmacy, Wuhan University in 2006. He then joined Prof. Jianmin Yue’s group at the Shanghai Institute of Materia Medica, Chinese Academy of Sciences for graduate study and performed research in bioactive natural product discovery. After receiving the Ph.D. degree at 2011, he went on to do postdoctoral work at University of Illinois at Chicago and University of Illinois at Urbana-Champaign under the supervision of Prof. Guido Pauli/Shaonong Chen and Prof. Satish Nair, respectively. His research mostly focuses on deciphering the catalytic mechanism of novel enzymes involved in bioactive natural product biosynthesis. Prof. Shihui Dong joined the Lanzhou University faculty at Nov. 2018 and has research interests that span the interface of chemistry and biology.

Representative Publications

1. “Mechanistic Basis for Ribosomal Peptide Backbone Modifications.” Shi-Hui Dong,1 Andi Liu,1 Nilkamal Mahanta, Douglas A. Mitchell,* and Satish K. Nair*. ACS Cent. Sci. 2019, DOI: 10.1021/acscentsci.9b00124.

2. “Biosynthesis of the RiPP Trojan Horse Antibiotic Microcin C is Directed by the N-Formyl Peptide Substrate.” Shi-Hui Dong, Alexey Kulikovsky, Svetlana Dubiley, Konstantin Severinov*, and Satish K. Nair*. Chem. Sci. 2019, 10, 2391-2395.

3. “The Biochemistry and Structural Biology of Cyanobactin Pathways: Enabling Combinatorial Biosynthesis.” Wenjia Gu, Shi-Hui Dong, Snigdha Sarkar, Satish K Nair*, Eric W Schmidt*. Methods in Enzymology 2018, 604, 113-163.

4. “Enzymatic reconstitution of ribosomal peptide backbone thioamidation.” Nilkamal Mahanta, Andi Liu, Shihui Dong, Satish K Nair*, Douglas A Mitchell*. PNAS 2018, 12, 3030-3035.

5. “Structural and Biochemical Studies of Non-native Agonists of the LasR Quorum-Sensing Receptor Reveal an L3 Loop "Out" Conformation for LasR.” Matthew C. O’Reilly,1 Shi-Hui Dong,1 Francis M. Rossi, Kaleigh M. Karlen, Rohan S. Kumar, Satish K. Nair*, and Helen E. Blackwell*. Cell Chem. Biol. 2018, 20, 1128-1139

6. “Molecular basis for the substrate specificity of quorum signal synthases.” Shi-Hui Dong, Nicole Frane, Quin H. Christensen, E. Peter Greenberg, Rajesh Nagarajan, and Satish K. Nair*. PNAS 2017, 114, 9092-9097.

7. “The enterococcal cytolysin synthetase has an unanticipated lipid kinase fold.” Shi-Hui Dong,1 Weixin Tang,1 Tiit Lukk, Satish K Nair, and Wilfred A van der Donk. eLife 2015, DOI: 10.7554/eLife.07607.

8. “Applications of the class II lanthipeptide protease LicP for sequence-specific, traceless peptide bond cleavage.” Weixin Tang,1 Shi-Hui Dong,1 Lindsay M. Repka, Chang He, Satish K Nair*, and Wilfred A van der Donk*. Chem. Sci. 2015, 6, 6270-6279.

9.  “Prolyl isomerase Pin1 regulates the stability, transcriptional activity and oncogenic potential of Brd4.” XiangMing Hu, Shi-Hui Dong, Jinjing Chen, Ruichuan Chen, Satish K Nair, Kun Ping Lu, and Lin-Feng Chen*. Oncogene 2017 doi:10.1038/onc.2017.137.

10.  “The Pimeloyl-CoA Synthetase BioW Defines a New Fold for Adenylate-Forming Enzymes.” Paola Estrada, Miglena Manandhar, Shi-Hui Dong, Jaigeeth Deveryshetty, Vinayak Agarwal, John E. Cronan, and Satish K. Nair*. Nat. Chem. Biol. 2017, 13, 668-674.


Room 3033, the First Chemistry Building




20022006Wuhan UniversityB.S.

20062011Shanghai Institute of Materia Medica, Chinese Academy of SciencesPh.D.